Additional file 1 of The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens

Additional file 1: Dose finding studies for phenotyping of DF-1 cells. Confluent DF-1 cells were infected at different MOI (0.1, 0.01, 0.001) and subsequently collected and stained for flow cytometrical analysis. A. After Life-dead staining with Zombie Aqua single, living cells were selected. Statistical analysis was done as two-way ANOVA with Tukey’s multiple comparison test. B. Living cells were further characterized with a monoclonal NP antibody. Statistical analyses were performed using two-way ANOVA with Šídák’s multiple comparisons test.