Additional file 1 of MiR-1248: a new prognostic biomarker able to identify supratentorial hemispheric pediatric low-grade gliomas patients associated with progression
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posted on 2022-06-19, 03:25 authored by Giuseppina Catanzaro, Zein Mersini Besharat, Andrea Carai, Natalie Jäger, Elena Splendiani, Carole Colin, Agnese Po, Martina Chiacchiarini, Anna Citarella, Francesca Gianno, Antonella Cacchione, Evelina Miele, Francesca Diomedi Camassei, Marco Gessi, Luca Massimi, Franco Locatelli, David T. W. Jones, Dominique Figarella-Branger, Stefan M. Pfister, Angela Mastronuzzi, Felice Giangaspero, Elisabetta FerrettiAdditional file 1: Figure S1. Overview of the methodological workflow of the study for the identification of miR-1248 as a progression risk stratification biomarker in pLGGs. Figure S2. Progression free survival (PFS) data for pLGG patients of cohorts I and II (p-value<0.001). Figure S3. (a) Normalized relative Ct expression levels of miR-376a-3p in pLGG without and with progression samples from cohort I (*p-value=0.025). (b) Log2 expression levels of miR-376a-3p in PA without and with progression samples from cohort II (*p-value=0.028). (c) Normalized relative Ct expression levels of miR-888-5p in pLGG without and with progression samples from cohort I (*p-value=0.007). (d) Log2 expression levels of miR-888-5p in PA without and with progression samples from cohort II (*p-value=0.007). (e) Normalized relative Ct expression levels of miR-1248 in pLGG without and with progression samples from cohort I (*p-value= 0.029). (f) Log2 expression levels of miR-1248 in PA without and with progression samples from cohort II (*p-value=0.0119). Figure S4. ROC curve of miR-376a-3p in pLGG with and without progression (AUC=0.5891; p-value=0.3986). Blue line=sensitivity, red line=identity. Figure S5. MiR-1248 levels distinguish Hemispheric PA and non-PA tumours with progression from Midline ones. (a) ddPCR copies/μL of miR-1248 in all the pLGG subgroups with progression. * p<0.05 vs PA Hemispheric with progression, ° p<0.05 vs GG Hemispheric with progression (b) ddPCR copies/μL of miR-1248 in Hemispheric PA with progression vs Midline PA with progression. ** p<0.01 vs PA Hemispheric with progression (c) ddPCR copies/μL of miR-1248 in Hemispheric non-PA with progression vs Midline non-PA with progression. The blue dots refer to hemispheric GG with progression, the fuchsia dot refers to the hemispheric DNET with progression, the turquoise dots refer to the midline GG with progression. * p<0.05 vs non-PA Hemispheric with progression.
