Additional file 1 of Lipocalin 2 induces neuroinflammation and blood-brain barrier dysfunction through liver-brain axis in murine model of nonalcoholic steatohepatitis

Additional file 1: Supplementary Fig. 1. A. Real time PCR expression of TNFα, IL-1β, MMP 9, VCAM1, ICAM 1 with brain endothelial cells exposed with Vehicle (CONTROL), Mouse recombinant Lcn2, Mouse recombinant leptin, and with Lcn2 and leptin both. Data was normalized with 18S rRNA expression and fold change was calculated with respect to CONTROL. Significance was calculated by one way ANOVA ( *** p < 0.001). B. Bar graph of normalized expression of ICAM1 and VCAM1, represented as ratio (ICAM1/VCAM1) of A. Data was represented as mean ± SD (n = 3), Significance was calculated by unpaired t-test with respect to CONTROL, * p < 0.05, ** p < 0.01, *** p < 0.001. Supplementary Fig. 2. A. Claudin 5 immunoreactivity displayed by immunoblot in mouse primary brain endothelial cells and 24p3RsiRNA exposed brain endothelial cells followed by treating with mouse recombinant Lcn2 and serum from MCD fed mouse group, and with vehicle (0.05% DMSO). B. Densitometric quantification of western blot in D, data was normalized with β actin, and expressed as mean ± SD, significance was calculated by paired t test between the groups, * p < 0.05, ** p < 0.01, *** p < 0.001.