Additional file 1 of Isolation and characterization of antibody fragments selective for human FTD brain derived TDP-43 variants

Additional file 1: Figure S1. Western blot under denaturing conditions. Reactivity against healthy control tissue and TDP-43 immunoprecipitated from healthy controls and FTD was assessed under reducing and denaturing conditions with A) Commercial TDP antibody, and B) FTD-TDP2 scFv. While commercial antibody recognizes TDP variants in FTD and healthy controls, FTD-TDP2 scFv does not recognize TDP variants in any of the samples. Figure S2. Western blot under native conditions. Reactivity against healthy control tissue and TDP-43 immunoprecipitated from healthy controls and FTD was assessed under non-reducing conditions with A) Commercial TDP antibody, and B) FTD-TDP2 scFv. While commercial antibody recognizes TDP variants in FTD and healthy controls, FTD-TDP2 scFv does not recognize TDP variants in any of the samples.