Additional file 1 of Comparative transcriptomic analysis identifies distinct molecular signatures and regulatory networks of chondroclasts and osteoclasts

Additional file 1 : Supplementary Figure 1. Quality of RNA isolated from LCM captured TRAP positive chondroclasts and osteoclasts as assessed by Agilent Bioanalyzer. The average size of the RNA fragments was between 100 to 150 bp maxing out at around 2 kb fragments. The majority though was within the 150 nt. Supplemental Figure 2. Immunofluorescent staining of differentially expressed genes showing co-localization of expression in TRAP-positive cells. Nucleus staining was performed to visualize the nucleus, whereas osteoclasts and chondroclasts were shown by TRAP staining. The immunostaining was performed using validated antibodies against Lmp2, Psat1, Nxn, Ufsp2 and Wnt5a. Supplementary Figure 3. Topological properties of PPI network of genes up-regulated in chondroclasts. Network analysis of PPI network using several topological features including (A) Betweenness Centrality (B) Topological Coefficients (C) Neighborhood Connectivity Distribution and (D) Closeness Centrality.