Additional file 1 of A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells

Matsufuji, Shohei; Kitajima, Yoshihiko; Higure, Kazuki; Kimura, Naoya; Maeda, Sachiko; Yamada, Kohei; Ito, Kotaro; Tanaka, Tomokazu; Kai, Keita; Noshiro, Hirokazu

doi:10.6084/m9.figshare.24779208.v1

40170_2023_328_MOESM1_ESM.pptx (1.82 MB)

Additional file 1 of A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells

presentation

posted on 2023-12-09, 04:41 authored by Shohei Matsufuji, Yoshihiko Kitajima, Kazuki Higure, Naoya Kimura, Sachiko Maeda, Kohei Yamada, Kotaro Ito, Tomokazu Tanaka, Keita Kai, Hirokazu Noshiro

Additional file 1: Supplementary Fig. S1. Flow cytometric analysis of apoptosis with double staining of Annexin V and PI. HepG2 and Hep3B cells were treated with PA at 0, 50, 100, and 200 μM concentrations under hypoxia for 48 hours. Hepatocellular carcinoma (HCC) cells not treated with PA under normoxia were also analyzed as a control. Detection of Annexin V−/PI+ or Annexin V+/PI+ was considered as apoptotic cells. The population was indicated by percentage.