13045_2017_421_MOESM1_ESM.pptx (109.44 kB)

Additional file 1: Figure S2. of Ovatodiolide suppresses colon tumorigenesis and prevents polarization of M2 tumor-associated macrophages through YAP oncogenic pathways

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posted on 28.02.2017, 05:00 by Yan-Jiun Huang, Ching-Kuo Yang, Po-Li Wei, Thanh-Tuan Huynh, Jacqueline Whang-Peng, Tzu-Ching Meng, Michael Hsiao, Yew-Ming Tzeng, Alexander Wu, Yun Yen
YAP1 expression is associated with M1-M2 polarization. (A) YAP1 silencing in macrophages (M0) leads to decreased M2 polarization. Real-time PCR analysis demonstrates that M2 markers, TGF-b1 and Ym2, were significantly reduced while one of the M1 marker iNOS was significantly increased, in the macrophages generated in the presence of DLD-1 cells. (B) YAP1-overexpressing colon cancer cells also promote the polarization of M2 genotype in THP-1 cells. Experiments were performed at least three times. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. The primer sequences used in the q-PCR experiments are shown. (PPTX 109 kb)


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