posted on 2018-07-31, 05:00authored byAmna Abu Khamidakh, Alejandra Rodriguez-Martinez, Kai Kaarniranta, Anne Kallioniemi, Heli Skottman, Jari Hyttinen, Kati Juuti-Uusitalo
Additional file 3: Video S2. The video corresponds Fig. 7C, D. Calcium wave in hESC-RPE monolayer (28d + 7d) followed for 300 s after mechanical stimulation. Prior the stimulation hESC-RPEs were loaded with fluorescent Ca2+ sensitive dye Fluo-4 AM that reflects [Ca2+]i concentration in the cytoplasm. The site of mechanical stimulation is marked with white an arrow. Mechanical stimulation of a single cell in a hESC-RPE monolayer resulted in a [Ca2+]i increase, seen as an increase in fluorescent signal, in the stimulated cell that propagates in a wave-like manner to neighbouring cells.
Funding
Luonnontieteiden ja Tekniikan Tutkimuksen Toimikunta