Additional file 2 of TREM2 Alzheimer’s variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages

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posted on 2020-11-17, 04:50 authored by Hazel Hall-Roberts, Devika Agarwal, Juliane Obst, Thomas B. Smith, Jimena Monzón-Sandoval, Elena Di Daniel, Caleb Webber, William S. James, Emma Mead, John B. Davis, Sally A. Cowley

Additional file 2. : Video 1. Time-lapse video of dead SH-SY5Y phagocytosis assay. WT pMac phagocytosing pHrodo-labelled fixed SH-SY5Ys, displaying an increase in red fluorescence of the SH-SY5Ys after engulfment. In 96wp, 2 x 104 pMac were given 2 x 104 SH-SY5Ys, and images taken every 10 minutes at 20x for 3 hours, using an EVOS FL Auto automated microscope. Video is 2 frames per second.

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Alzheimer’s Research UK Oxford Drug Discovery Institute Oxford Martin School, University of Oxford Parkinson’s UK Medical Research Council Wellcome Trust Wellcome Trust

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TREM2 R47H Alzheimer’s disease iPSC Human pluripotent stem cells Macrophages Microglia Phagocytosis Inflammation Synaptosome

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