MOESM9 of SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding
journal contributionposted on 14.01.2020 by Qingxiu Liu, Wei Zhang, Siying Chen, Zhenjing Zhuang, Yi Zhang, Lingli Jiang, Jun LIN
Any type of content formally published in an academic journal, usually following a peer-review process.
Additional file 9: Figure S8. Generation and characterization of aptamers against DDA. A, SELEX protocol to isolation of aptamers bound to DDA; B, Enrichment of the dynamic libraries in SELEX rounds by real-time PCR; C and D, characterization of the affinity (C) and selectivity (D) of one aptamer against DDA by using SYBR Green I assay. BB:Binding buffer; DDA: 2,2-bis(4-chlorophenyl) Acetic Acid; DDT: 2,4′-DDT; PFOA: Perfluorooctanoic Acid; EP: Ethyl pyruvate; TBBPA: Tetrabromobisphenol A; Data represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.