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MOESM9 of SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding

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posted on 14.01.2020 by Qingxiu Liu, Wei Zhang, Siying Chen, Zhenjing Zhuang, Yi Zhang, Lingli Jiang, Jun LIN
Additional file 9: Figure S8. Generation and characterization of aptamers against DDA. A, SELEX protocol to isolation of aptamers bound to DDA; B, Enrichment of the dynamic libraries in SELEX rounds by real-time PCR; C and D, characterization of the affinity (C) and selectivity (D) of one aptamer against DDA by using SYBR Green I assay. BB:Binding buffer; DDA: 2,2-bis(4-chlorophenyl) Acetic Acid; DDT: 2,4′-DDT; PFOA: Perfluorooctanoic Acid; EP: Ethyl pyruvate; TBBPA: Tetrabromobisphenol A; Data represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

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