MOESM7 of Enhancing the toolbox to study IL-17A in cattle and sheep

Additional file 7. Principal components analysis biplot of binding of commercial antibodies to transfected Chinese Hamster Ovary cells stably-expressing recombinant bovine or ovine IL-17A. A principal component analysis (PCA) was conducted to investigate the structure of relationships between commercial monoclonal antibody clones to IL-17A and the six metrics used to assess antibody staining. These metrics were the binding to transfected CHO cells stably expressing rbovIL-17A, rovIL-17A or the untransfected CHO negative control (UTF) cells [numerical percentage of (%) cells] in the upper positive region (% CHO UTF, % bovIL-17A and % ovIL-17A) and delta median fluorescence intensity (deltaMFI) values for the same three CHO cell lines. Data used in the PCA is taken from Additional File 6. PCA reduced the dimension of the data set by means of optimal linear combinations (principal components, PCs) of the six metrics aimed to retain as much of the original data variability as possible. The results were displayed using a correlation biplot based on the two first PCs (those accounting for the highest percentage of the total variability) to facilitate discussion and ranking of the commercial antibodies.