12936_2018_2369_MOESM6_ESM.pdf (188.16 kB)
MOESM6 of Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies
journal contribution
posted on 2018-06-01, 05:00 authored by Itziar Ubillos, Alfons Jiménez, Marta Vidal, Paul Bowyer, Deepak Gaur, Sheetij Dutta, Benoit Gamain, Ross Coppel, Virander Chauhan, David Lanar, Chetan Chitnis, Evelina Angov, James Beeson, David Cavanagh, Joseph Campo, Ruth Aguilar, Carlota DobañoAdditional file 6. Levels of IgG and IgG2-4 to 15 antigens measured in the WHO reference reagent compared to negative control and blanks under three different incubation conditions. Curve plots of the antigen-specific antibody levels measured in serial dilutions of the WHO reference reagent, negative control and blanks at three different incubation conditions: 37 °C 2 h (37 °C 2 h), 4 °C overnight (4 °C ON) and room temperature for 1 h (RT 1 h). “neg” means negative control.