13567_2018_546_MOESM3_ESM.pdf (272.93 kB)
MOESM3 of RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois
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posted on 2018-06-18, 05:00 authored by Naíla Nascimento, Ana Guimaraes, Andrea Santos, Yuefeng Chu, Lucas Marques, Joanne MessickAdditional file 3. Relative expression (RE) of differentially expressed (DE) in pigs infected with Mycoplasma suis validated by qRT-PCR. Relative expression profile (GAPDH as reference control gene) of DE genes identified in the M. suis-infected pigs compared to the control group (non-infected). Validated genes are: ISG15 ubiquitin-like modifier (ISG15), interleukin 22 receptor, alpha 2 (IL22RA2), CD274 molecule (CD274), BCL2-like 14 (apoptosis facilitator) (BCL2), chemokine (C-C motif) receptor 5 (CCR5), CD180 molecule (CD180), allograft inflammatory factor 1 (AIF1), interleukin 15 (IL15), protein tyrosine phosphatase, receptor type, O (PTPRO), and Toll-like receptor 8 (TLR8). Black dots in each graphic represent the RE of a respective gene for each pig; blue vertical lines represent the standard deviation in each group. * p-value ≤ 0.1, and ** p-value ≤ 0.05.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico
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p-valueRECCRsuis-infected pigsreceptor 5control groupGAPDHinterleukinDE genesMycoplasma suisMOESM 3IL 22RA CD 274 moleculeBCLRelative expression profileSus scrofareceptor typeimmunocompetent pigsreference control genePTPROToll-like receptor 8TLRMycoplasma suis strain IllinoisC-C motiffactor 1AIFValidated genesISG 15 ubiquitin-like modifierapoptosis facilitator
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