13567_2016_390_MOESM3_ESM.pdf (184.21 kB)
MOESM3 of Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09
journal contribution
posted on 2016-10-20, 05:00 authored by Johanneke Hemmink, Sophie Morgan, Mario Aramouni, Helen Everett, Francisco Salguero, Laetitia Canini, Emily Porter, Margo Chase-Topping, Katy Beck, Ronan Loughlin, B. Carr, Ian Brown, Mick Bailey, Mark Woolhouse, Sharon Brookes, Bryan Charleston, Elma TchilianAdditional file 3. Principal component analysis for IFNγ ELISPOT, ICS and Ab titers. PC loadings for PC1 and PC2 (PC2 was not significantly associated with treatment group) showing the amount of variability in PC1 and PC2 that can be explained by each variable. Intracellular cytokine production by PBMCs was positively loaded on PC1, while IFN-γ production by cells in the ELISPOT assay was negatively loaded. BAL samples cluster together and are also negatively loaded. Antibody levels in BAL and serum correlate with IFN-γ secretion by cells in the BAL and with IFN-γ production by CD4+CD8+ T cells in LN.
