MOESM3 of Development of TaqMan probes targeting the four major celiac disease epitopes found in Îą-gliadin sequences of spelt (Triticum aestivum ssp. spelta) and bread wheat (Triticum aestivum ssp. aestivum)

Additional file 3. Primer efficiency calculation for the four T-cell stimulatory epitopes involved in CD and for the four reference genes used to normalize the epitope expression levels. The file presents the efficiency calculation results obtained for the four most stable reference genes used in this study to normalize epitope expression levels.