13099_2019_295_MOESM1_ESM.pdf (1.42 MB)
MOESM1 of Quantification of serine protease HtrA molecules secreted by the foodborne pathogen Campylobacter jejuni
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posted on 2019-04-12, 05:00 authored by Matthias Neddermann, Steffen BackertAdditional file 1: Figure S1. Purification of recombinant C. jejuni HtrA expressed in E. coli BL21. Important purification steps are shown, including purification by use of a GST-affinity column, exemplary wash and two elution fractions. The final purification step was performed via gel filtration, showing purified HtrA in fraction 2.
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