12862_2019_1543_MOESM1_ESM.pdf (5.69 MB)
MOESM1 of Human-specific mutations in VMAT1 confer functional changes and multi-directional evolution in the regulation of monoamine circuits
journal contribution
posted on 2019-12-03, 04:42 authored by Daiki Sato, Yuu Ishii, Tomoaki Nagai, Kazumasa Ohashi, Masakado KawataAdditional file 1: Figure S1. (a) Full-length blots for Fig. 2. (b) Four additional experiments of immunoblotting for YFP-VMAT1 with anti-VMAT1 and anti-α-tubulin antibodies. (c) Relative expression levels of each variant of VMAT1 corrected by α-tubulin based on immunoblotting. No significant differences were observed among variants in either comparison (P-values calculated by one-way ANOVA: 0.99).