MOESM1 of Application of a New Multiplexed Array for Rapid, Sensitive, Simultaneous and Quantitative Assessment of Spliced and Unspliced XBP1
journal contributionposted on 15.11.2019, 05:25 by Stuart Creedican, Aaron Talty, Stephen Fitzgerald, Afshin Samali, CiarÃ¡n Richardson, Adrienne Gorman, Kenneth Martin
Additional file 1: Figure S1. A sandwich immunoassay which captured the conventionally (conv.) spliced or frameshifted C-terminus of the XBP1 isoforms and a pan-detector of the common N-terminus was designed. Due to the unconventional splicing of XBP1 mRNA a translational frameshift occurs and results in XBP1 isoforms of differing length and C-terminal sequences (A). A biochip was proposed that utilised these different C-termini for simultaneous capture and the common N-terminus for detection (B).