MOESM1 of A Penicillium chrysogenum-based expression system for the production of small, cysteine-rich antifungal proteins for structural and functional analyses
journal contribution
posted on 2016-11-11, 05:00 authored by Christoph Sonderegger, László Galgóczy, Sandra Garrigues, Ádám Fizil, Attila Borics, Paloma Manzanares, Nikoletta Hegedüs, Anna Huber, Jose Marcos, Gyula Batta, Florentine MarxAdditional file 1. Table S1. Composition of media used in this study. Table S2. Oligonucleotides used in this study. Figure S1. Nucleotide sequence of plasmid pSK275paf containing the paf expression cassette. Figure S2. Cloning strategy of pSK275nfap paf_signal . Figure S3. Southern blot analysis of genomic integration of the P. chrysogenum expression cassettes in the Penicillium spp. Figure S4. Chromatogram of the purification of PAF[Pd]. Figure S5. Microscopic images of growth inhibition assays with A. niger exposed to PAF, PAF[Pc], PAFF31N, PAFY48Q, PAF[Pd], NFAP and NFAP[Pc].
