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Additional file 9 of Elucidating the role of the gut microbiota in the physiological effects of dietary fiber

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posted on 14.05.2022, 05:10 by Edward C. Deehan, Zhengxiao Zhang, Alessandra Riva, Anissa M. Armet, Maria Elisa Perez-Muñoz, Nguyen K. Nguyen, Jacqueline A. Krysa, Benjamin Seethaler, Yuan-Yuan Zhao, Janis Cole, Fuyong Li, Bela Hausmann, Andreas Spittler, Julie-Anne Nazare, Nathalie M. Delzenne, Jonathan M. Curtis, Wendy V. Wismer, Spencer D. Proctor, Jeffrey A. Bakal, Stephan C. Bischoff, Dan Knights, Catherine J. Field, David Berry, Carla M. Prado, Jens Walter
Additional file 9: Figure S3. Confirmation of gut microbiota compositional features and mechanistic biomarkers that predict clinical responses. Line graphs show differences in the effects of A AX on perceived satiety after a meal and HOMA-IR and B MCC on fecal calprotectin and TNF-α for high and low responders, as defined according to the study cohort median. AUC-ROC values show the performance accuracy of random forest classifiers for predicting high-vs-low responders in C AX-induced perceived satiety after a meal and HOMA-IR attenuation, D MCC-induced fecal calprotectin and TNF-α attenuation, and E AX and MCC induced changes in HOMA-IR, fecal calprotectin, and TNF-α. High and low responders were defined according to the study cohort median. Black cells denote OOB error rates ≥ 0.6. Prediction performance of random forest classifiers trained to predict high-vs-low responders in AX-induced F and G satiety after a meal and H HOMA-IR attenuation, and MCC-induced I fecal calprotectin and J TNF-α attenuation. OOB shows the mean prediction error of the random forests model with boosted decision trees (n = 500). The confusion matrix shows subgroup prediction accuracy, where row i and column j indicates the number of subjects predicted as i but were actually classified as j. Error rates indicate the percentage of incorrect classifications. ∆, absolute change from baseline to week 6; %∆, percent change from baseline to week 6; ASV, amplicon sequence variant; AX, arabinoxylan; AUC-ROC, area under the receiver operating characteristic curve; HOMA-IR, homeostatic model assessment of insulin resistance; MCC, microcrystalline cellulose; OTU, operational taxonomic unit; OOB: out-of-bag; SLIM, Satiety Labeled Intensity Magnitude; TNF-α, tumor necrosis factor-α.

Funding

European Research Council Joint Programming Initiative A healthy diet for a healthy life Science Foundation Ireland Centre

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