Additional file 8 of WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice

Additional file 8: Figure S5. RNA-seq analysis of WT and wsl9 seedlings at 30 °C. mRNA was enriched from total RNA isolated from 10-d-old WT and wsl9 mutant seedlings using oligo-(dT) fragmented and reverse-transcribed using random hexamer primers. The library was then constructed and sequenced using an Illumina HiSEquation 2000. a Frequencies of detected genes sorted according to expression level. b Read numbers of WT and wsl9 mutant sequences. c Volcano plot showing overall alterations in gene expression in WT and wsl9 mutant.