12943_2020_1223_MOESM7_ESM.pdf (1.11 MB)
Additional file 7 of m6A modification-mediated BATF2 acts as a tumor suppressor in gastric cancer through inhibition of ERK signaling
journal contribution
posted on 2020-07-11, 16:07 authored by Jian-Wei Xie, Xiao-Bo Huang, Qi-Yue Chen, Yu-Bin Ma, Ya-Jun Zhao, Li-Chao Liu, Jia-Bin Wang, Jian-Xian Lin, Jun Lu, Long-Long Cao, Mi Lin, Ru-Hong Tu, Chao-Hui Zheng, Chang-Ming Huang, Ping LiAdditional file 7: Figure S2. The effect of BATF2 expression on GC cell proliferation. a MGC-803 and HGC-27 cells with stable BATF2 overexpression or knockdown were created. The changes in BATF2 expression were confirmed using western blotting. b Colony formation and CCK-8 assays with stably transfected MGC-803 cells were performed. Representative images and quantification of the results are presented (**P < 0.01; ***P < 0.001). c Colony formation and CCK-8 assays with stably transfected HGC-27 cells were performed. Representative images and quantification of the results are presented (*P < 0.05; **P < 0.01). d Representative images of Ki-67 IHC staining in xenografted tumors. Scale bars = 200 μm. The Ki-67 IHC scores are shown in the indicated tissues (**P < 0.01).