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Additional file 7: of Hypoxia-induced Slug SUMOylation enhances lung cancer metastasis

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posted on 06.01.2019, 05:00 by Pei-Fang Hung, Tse-Ming Hong, Che-Chang Chang, Chung-Lieh Hung, Yuan-Ling Hsu, Yih-Leong Chang, Chen-Tu Wu, Gee-Chen Chang, Nei-Li Chan, Sung-Liang Yu, Pan-Chyr Yang, Szu-Hua Pan
Figure S7. Slug recruits corepressors more abundantly than Slug5M. The nuclear fractions of Slug- and Slug5M-overexpressing HEK293 cells were obtained by adding hypotonic buffer to the cells. Subsequently, the samples were subjected to immunoprecipitation using an anti-Slug antibody. The accompanying precipitates were analyzed by immunoblotting using the indicated antibodies (left panel). Lamin B was used as a nuclear marker. The relative densitometry results (the results for Slug were normalized to one) were calculated using two programs (ImageJ and GelPro3.1), and the average values are plotted in the right panel. (PDF 61 kb)

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Ministry of Science and Technology, Taiwan

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