40168_2022_1248_MOESM7_ESM.pdf (69.92 kB)
Download file

Additional file 7 of Elucidating the role of the gut microbiota in the physiological effects of dietary fiber

Download (69.92 kB)
journal contribution
posted on 14.05.2022, 05:10 by Edward C. Deehan, Zhengxiao Zhang, Alessandra Riva, Anissa M. Armet, Maria Elisa Perez-Muñoz, Nguyen K. Nguyen, Jacqueline A. Krysa, Benjamin Seethaler, Yuan-Yuan Zhao, Janis Cole, Fuyong Li, Bela Hausmann, Andreas Spittler, Julie-Anne Nazare, Nathalie M. Delzenne, Jonathan M. Curtis, Wendy V. Wismer, Spencer D. Proctor, Jeffrey A. Bakal, Stephan C. Bischoff, Dan Knights, Catherine J. Field, David Berry, Carla M. Prado, Jens Walter
Additional file 7: Figure S2. Sorting of AX-stimulated bacterial cells by FACS on a MoFlow Astrios EQ cell sorter. As shown in the dot plots, A background noise of the machine was detected using FSC and SSC parameters. B Bacterial cells were measured in the same setting and pre-gated. C An example of Cy5-negative cells is presented in the dot plot showing the Cy5 channel via the SSC channel. D An example of Cy5-positive fluorescent cells (activated by AX) that were gated and sorted out by FACS. AX, arabinoxylan; FACS, fluorescence-activated cell sorting; FSC, forward scatter; SSC, side scatter.

Funding

European Research Council Joint Programming Initiative A healthy diet for a healthy life Science Foundation Ireland Centre

History

References