40168_2021_1180_MOESM5_ESM.docx (805.51 kB)
Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)
journal contribution
posted on 2021-11-26, 05:15 authored by Kevin Xu Zhong, Anna Cho, Christoph M. Deeg, Amy M. Chan, Curtis A. SuttleAdditional file 5: Figure S2. Eukaryotic taxa representing >1% of the sequences revealed by deep-sequencing of the 18S rRNA amplicons for oyster spat samples, using "universal" 18S primers (Table S3), non-metazoan primers (Table S3), blocking primers (Table S3), or CRISPR-Cas Selective Amplicon Sequencing (CCSAS) combining "universal" 18S primers and CRISPR-Cas9 with pacific-oyster-specific sgRNA m258 (Table S2).
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Gordon and Betty Moore Foundation Pacific Research Board
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