Additional file 4: of Zebrafish prdm12b acts independently of nkx6.1 repression to promote eng1b expression in the neural tube p1 domain

Characterization of the prdm12bsa9887 mutant. a. Schematic showing genomic sequence of prdm12b. Exons are indicated as boxes and black lines represent introns. The PR domain and three zinc fingers (ZnF) are highlighted in dark red and blue, respectively. The black arrow indicates a single base pair change in the second intron of prdm12bsa9887. b, c. Sequence traces confirming the expected single nucleotide change in wildtype (b) versus prdm12b+/sa9887 (c) animals. d-g. eng1b expression in 24hpf embryos from a cross of prdm12b+/sa9887 animals. Embryos are shown in dorsal (d, e) or lateral (f, g) view with anterior to the left. eng1b expression is lost in 27% of embryos compared to 73% of embryos showing wildtype eng1b staining. (PDF 1962 kb)