13075_2021_2670_MOESM4_ESM.docx (317.31 kB)
Additional file 4 of WNT16 elevation induced cell senescence of osteoblasts in ankylosing spondylitis
journal contribution
posted on 2021-12-09, 04:26 authored by Sungsin Jo, Subin Weon, Bora Nam, Mi-Ae Jang, Hyundeok Kang, Tae-Jong Kim, Ye-Soo Park, Tae-Hwan KimAdditional file 4: Supplementary Figure 4. WNT16 treatment inhibited ALP activity in AS-osteoprogenitors. AS-osteoprogenitors were plated in 96 wells plate and treated with 0, 25, or 50 ng/ml WNT16, and then analyzed as indicated by (A) WST assay for cell proliferation. (B) ALP and COL staining, and (C) ALP activity. Data are presented as the mean ± SD (n=3). *p<0.05.
Funding
the national research foundation of korea
History
Usage metrics
Categories
Keywords
osteoblast differentiation accompaniedassociated secretory phenotypeprimary osteoprogenitor cellsosteoprogenitor cells comparedwnt16 knockdown culturessasp mrna expressionisolated osteoprogenitor cellsinduced sasp expressionassess cell senescenceseven disease controlosteoprogenitor cellswnt16 expressionisolated usingcell senescencewnt16 treatmentvitro modelvitro assaysstill unknownstained markedlypathophysiological changesp21 proteinoutgrowth methodidentified rolehighly expressedh2o2 stressforming activityfacet jointsconclusion wnt16ankylosing spondylitisalso validated
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC