Additional file 4: of TREM2 deficiency exacerbates tau pathology through dysregulated kinase signaling in a mouse model of tauopathy

A,B Western blot analysis reveals no alterations in cortical or hippocampal signaling molecules between 6-month non-transgenic BL6 control mice and Trem2 −/− mice. Additionally, no significant alterations in p-tau (C) were detected between BL6 and Trem2 −/− genotypes. Modest non-significant increases in total tau (Tau5) were detected in Trem2 −/− mice compared to BL6 non-transgenic mice. (D) Morphological analysis of Iba1 staining demonstrates no alterations among microglia between BL6 and Trem2 −/− control mice (N = 4 mice per genotype). At least two independent experiments were performed for each analysis. Error bars represent SEM. *, P < 0.05, **, P < 0.01, ***, P < 0.001. (PDF 1980 kb)