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Additional file 4: of Hypoxia-induced Slug SUMOylation enhances lung cancer metastasis

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journal contribution
posted on 06.01.2019, 05:00 by Pei-Fang Hung, Tse-Ming Hong, Che-Chang Chang, Chung-Lieh Hung, Yuan-Ling Hsu, Yih-Leong Chang, Chen-Tu Wu, Gee-Chen Chang, Nei-Li Chan, Sung-Liang Yu, Pan-Chyr Yang, Szu-Hua Pan
Figure S4. Direct interaction of Slug with PIAS family members. A pull-down assay was used to determine the physical interaction between Slug and PIAS family members. Recombinant GST and GST–Slug proteins were produced from bacteria, and the translated products of HA-tagged PIAS family member genes were obtained using an in vitro transcription/translation system. The production of these proteins was demonstrated by immunoblotting using anti-GST and anti-HA antibodies, respectively. GST–Slug was used in the pull-down assay for in vitro interaction with HA-tagged PIAS family members. The GST protein alone was used as a negative control. (PDF 24 kb)


Ministry of Science and Technology, Taiwan