Additional file 4 of Genome-wide identification and expression analysis of aquaporin family in Canavalia rosea and their roles in the adaptation to saline-alkaline soils and drought stress

Additional file 4: Figure S4. Salt, salt-alkaline, and high osmotic stress analyses of the transgenic plants with CrPIP1;5’s overexpression based on seedling root lengths. Four-day-old seedlings were transplanted into MS medium containing NaCl, NaCl plus NaHCO3 (pH 8.2) or mannitol and then grown for 7 d before measuring the root length. a Photographs of the transgenic lines (CrPIP1;5OX 1#, OX 5#, and OX 10#) and WT seedlings on MS medium or MS medium with NaCl, NaCl plus NaHCO3 (pH 8.2), or mannitol; b‒d The seedling root lengths (mm) in WT and the transgenic lines under NaCl (b), NaCl plus NaHCO3 (pH 8.2) (c), or mannitol (d) stresses after 7 d. Error bars indicate the SD based on over three replicates (n ≥ 3). Asterisks indicate significant differences from the control (Student’ s t-test P values, * p < 0.05 and ** p < 0.01).