40478_2020_988_MOESM4_ESM.pdf (1.28 MB)
Additional file 4 of Different effects of constitutive and induced microbiota modulation on microglia in a mouse model of Alzheimer’s disease
journal contribution
posted on 2020-07-30, 06:21 authored by Charlotte Mezö, Nikolaos Dokalis, Omar Mossad, Ori Staszewski, Jana Neuber, Bahtiyar Yilmaz, Daniel Schnepf, Mercedes Gomez de Agüero, Stephanie C. Ganal-Vonarburg, Andrew J. Macpherson, Melanie Meyer-Luehmann, Peter Staeheli, Thomas Blank, Marco Prinz, Daniel ErnyAdditional file 4: Supplementary Fig. 4. Microglial density in hippocampus of 4 months old 5xFAD mice. (A) Representative immunofluorescence images of Iba1+ (green) microglia on coronal hippocampal sections of 4 months old SPF, GF and ABX-treated WT mice. Nuclei were stained with DAPI (blue). Scale bar: 50 μm. (B) Representative immunofluorescence images of 6E10 (red) and Iba1 (green) on coronal hippocampal sections of 4 months old 5xFAD mice. Nuclei were stained with DAPI (blue). Scale bar: 50 μm. (C) Quantification of 6E10+ plaque-associated microglia. Each symbol represents one mouse. Data are represented as means ± s.e.m. Significant differences were determined by one-way ANOVA followed by Tukey’s post-hoc comparison test (**P < 0.01, ***P < 0.001). Data are representative of two independent experiments.
