Additional file 4: of DEPTOR induces a partial epithelial-to-mesenchymal transition and metastasis via autocrine TGFβ1 signaling and is associated with poor prognosis in hepatocellular carcinoma

Figure S1. Western blotting analysis of relative DEPTOR expression in 53 HCC tissues (T) and its adjacent non-tumor tissues (N). Figure S2. (A) Western blotting was used to detect the overexpression efficiency of 7402 and HepG2 cells. (B) Proliferation of 7402-DEP, HepG2-DEP cells and control cells were examined by CCK8 assay. (C) Proliferation of 7402-DEP, HepG2-DEP cells and control cells were examined by colony formation assay. Figure S3. (A) Representative phase contrast images of HepG2-DEP cells and their control cells. (B) IF for DEPTOR was shown in HLF-shDEP1/2 cells and their control cells. Scale bar: 30 μm. (C) Overexpression of snail expression promoted EMT in HLF-shDEP1 cells. (D) The transwell assay was used to detect the capacity of migration and invasion in the indicated cells following snail overexpression. (E) Representative images of IHC staining with anti-DEPTOR and anti-E-cadherin. The expression of DEPTOR was inversely correlated with that of E-cadherin. Scale bar: 300 μm (left panel) and 30 μm (right panel). The data represent means ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Figure S4. The sequences of a series of truncated or mutant DEPTOR 5′-promoter luciferase constructs. (DOCX 2973 kb)