Additional file 4 of AUY922 induces retinal toxicity through attenuating TRPM1

Additional file 4: Figure S4. a Representative western blot results of HSP90 and HSP70 co-IP with 3xF-TRPM1. Cell lysates were prepared from Mel1617 cells stably expressing either an empty vector or 3xF-TRPM1 and IP with anti-FLAG M2 affinity gel. n = 3. b Quantification analysis, related to Fig. 4b, was performed for three independent experiments. Data are presented as the mean ± s.e.m. The P values were determined by two-tailed Student’s t-test, * P < 0.05; ** P < 0.01. n = 3. c Quantification analysis, related to Fig. 4c, was performed for three independent experiments. Data are presented as the mean ± s.e.m. The P values were determined by two-tailed Student’s t-test, ** P < 0.01; *** P < 0.001. n = 3. d Quantification analysis, related to Fig. 4d, was performed for three independent experiments. Data are presented as the mean ± s.e.m. The P values were determined by two-tailed Student’s t-test, ** P < 0.01; n.s. = not significant. n = 3. e Representative western blot results of CDC37, 3xF-TRPM1 and HSP70 co-IP with HSP90. ARPE19 cells expressing 3xF-TRPM1 were mock-treated (-) or treated with 30 nM AUY922 for 4 h ( +). Cell lysates were prepared and IP with anti-HSP90 conjugated agarose. Mouse IgG antibodies were served as an IP control. Quantification was performed for three independent experiments. Data are presented as the mean ± s.e.m. The P values were determined by two-tailed Student’s t-test, ** P < 0.01; *** P < 0.001; n.s. = not significant. n = 3. f Quantification analysis, related to Fig. 4e, was performed for three independent experiments. Data are presented as the mean ± s.e.m. The P values were determined by two-tailed Student’s t-test, ** P < 0.01; n.s. = not significant. n = 3.