Additional file 3 of MxB impedes the NUP358-mediated HIV-1 pre-integration complex nuclear import and viral replication cooperatively with CPSF6

Additional file 3. Overexpression of CPSF6 decreases HIV-1 nuclear import but not viral infection in the presence of MxB. (a) HeLa-Ctrl and HeLa-MxB cells were not transfected (Mock) or transfected with plasmid expressing CPSF6-Flag protein or empty vector. 48 h after transfection, the expression levels of CPSF6 were monitored by western blot. (b, c) Transfected cells were then incubated with VSV-G pseudotyped HIV-1 luciferase reporter virus, infectivity was determined 48 h post infection by luciferase assay (b) and p24 protein expression (c). (d) qPCR analysis of HIV-1 Late RT DNA, 2-LTR circles and Integrated DNA was preformed 24 h post infection. Results were a summary of 3 independent experiments, an unpaired t test was performed (NS, not significant, *p < 0.05, **p < 0.01 and ***p < 0.001).