Additional file 3 of Lipopolysaccharide- TLR-4 Axis regulates Osteoclastogenesis independent of RANKL/RANK signaling

Additional file 3: Figure S3. Immunoblotting analysis of the effect of OPG treatment on RANK expression. (A) An equal amount of membrane lysate proteins were used for immunoblotting analyses with antibodies to RANK (~90 kDa) and GAPDH (loading control; ~37 kDa). Protein levels were quantified by densitometry, corrected for the sample load based on GAPDH expression, and expressed as fold-decrease relative to the control lanes (RANKL and LPS). The results represent one of three experiments performed. (B) Uncropped raw data for the immunoblotting analyses shown in panel A are provided. Red rectangle indicates the proteins that are shown in panel A. The other lanes that were not marked by a rectangle in each autoradiogram represent different treatments which are not pertinent to the present studies.