Additional file 3 of Establishment of an in vitro model for analyzing mitochondrial ultrastructure in PRKN-mutated patient iPSC-derived dopaminergic neurons

Additional file 3: Figure S2. Flow cytometric analysis of mitochondrial membrane potential in dopaminergic neurons derived from TH-GFP iPSCs. (a) Histograms showed GFP-negative and GFP-positive populations in the TH-GFP iPSC-derived differentiated cells. Data were obtained from three independent experiments. “PRKN” represents PRKN-mutated patient. Values are shown as the mean ± SEM. (b) TMRM-GFP cytograms (top) represented the intensity of TMRM signal in GFP-negative and -positive populations. Mitotracker DeepRed-GFP cytograms (bottom) represented the intensity of Mitotracker DeepRed signal in GFP-positive and -positive populations. “PRKN” represents PRKN-mutated patient. (c) Quantitative analysis of the MFI of TMRM (left), Mitotracker DeepRed (center), and TMRM normalized with Mitotracker DeepRed (right) in GFP-positive cells relative to GFP-negative cells in the control and PRKN-mutated lines. Data were obtained from three independent experiments. “PRKN” represents PRKN-mutated patient. Values are shown as the mean ± SEM. Statistical significance was evaluated using the unpaired two-tailed t-test. *P < 0.05. There was no significant difference in the MFI of Mitotracker between control GFP-positive and -negative cells (control center; P = 0.3502). There were no significant differences in the MFI of TMRM and TMRM/Mitotracker between PRKN-mutated GFP-positive and -negative cells (PRKN left; P = 0.5567, PRKN right; P = 0.1382).