Additional file 3 of Defective migration and dysmorphology of neutrophil granulocytes in atypical chronic myeloid leukemia treated with ruxolitinib

Additional file 3: Figure S3. Changes in leukocyte parameters during ruxolitinib therapy and correlation of peripheral leukocyte counts with migration patterns. (A) – (E) Time course of chosen peripheral blood parameters of the aCML patient over the course of his disease and therapy. Displayed are the absolute WBC (A), myeloblast (B) and metamyelocyte (C) counts in cells/nl as well as the banded neutrophil granulocyte (D) and myelocyte (E) counts relative to the overall WBC in %. Red dots indicate timepoints where migration and flow cytometry data were acquired. (F) Correlation of peripheral cell counts with migration patterns upon PBS stimulation. From left to right: leukocyte counts with moving cells (PBS), leukocyte counts with speed excluding non-moving cells (PBS), metamyelocytes with speed excluding non-moving cells (PBS) and banded neutrophils with speed excluding non-moving cells (PBS). (G) Correlation of metamyelocyte counts with speed excluding non-moving cells upon CXCL1 treatment. (H) Correlation of metamyelocyte counts with speed excluding non-moving cells upon CXCL8 treatment. (F) - (H) Correlations were computed using GraphPad Prism™. Spearman r and p-values are given for each correlation below the graph.