40709_2020_123_MOESM3_ESM.docx (13.69 kB)
Additional file 3 of Application of antibody phage display to identify potential antigenic neural precursor cell proteins
journal contribution
posted on 2020-08-03, 04:33 authored by Ioannis Paspaltsis, Evangelia Kesidou, Olga Touloumi, Roza Lagoudaki, Marina Boziki, Martina Samiotaki, Dimitra Dafou, Theodoros Sklaviadis, Nikolaos GrigoriadisAdditional file 3. PCR primers used in this study. Primers 1–19 were used for the amplification of IgG Fab fragments, while primers 20–21 for the sequencing of the isolated phagemids.Degenerative nucleotide symbols:K = G or T, S = C or G, M = A or C, W = A or T, R = A or G, Y = C or T. Restriction enzymes recognition sites are underlined, with A|CTAGT for SpeI, C|TCGAG for XhoI, T|CTAGA for XbaI and GAGCT|C for SacI.