13046_2021_1948_MOESM2_ESM.pdf (362.98 kB)
Additional file 2 of ZBTB28 induces autophagy by regulation of FIP200 and Bcl-XL facilitating cervical cancer cell apoptosis
journal contribution
posted on 2021-05-01, 03:28 authored by Li Li, Yijia Gong, Ke Xu, Weihong Chen, Jiuyi Xia, Zhaobo Cheng, Lili Li, Renjie Yu, Junhao Mu, Xin Le, Qin Xiang, Weiyan Peng, Junying Tang, Tingxiu XiangAdditional file 2: Figure S1. Nucleus localization of ZBTB28. The localization of ZBTB28 and vimentin or Ecad in the nucleus of HeLa was analyzed by immunofluorescence. Figure S2. Regulation of ZBTB28 on Bcl-2 family proteins. (A) The expression of BECN1 was analyzed. (B, C) The expression of BCL2 and MCL1 was analyzed by qRT-PCR. (D) Promoter luciferase activity of Bcl-XL in cervical cancer cells. Figure S3. ZBTB28 regulated ATG16L2 in cervical cancer cells. (A, B) qRT-PCR results of ATGs mRNA expression in stable transfected cervical cancer cells. (C) Structure of wild-type ATG16L2 reporter plasmid. (D) % input of ATG16L2 DNA by HA-tag antibody were detected by ChIP-PCR. (E) Use dual luciferase reporter system to detect the effect of ZBTB28 on ATG16L2 promoter activity in 293T, CaSki and HeLa cells.
