13075_2021_2670_MOESM2_ESM.docx (293 kB)
Additional file 2 of WNT16 elevation induced cell senescence of osteoblasts in ankylosing spondylitis
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posted on 2021-12-09, 04:26 authored by Sungsin Jo, Subin Weon, Bora Nam, Mi-Ae Jang, Hyundeok Kang, Tae-Jong Kim, Ye-Soo Park, Tae-Hwan KimAdditional file 2: Supplementary Figure 2. The result of siRNA efficiency test. (A) Total four human osteoblasts cell lines (MG63, FOB, SaOS2, U2OS) were extracted total RNA and analyzed by RT-PCR to test WNT16 level. (B) FOB cell were transfected with five siRNA oligo against human WNT16 and analyzed by RT-PCR for siRNA efficiency.
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the national research foundation of korea
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osteoblast differentiation accompaniedassociated secretory phenotypeprimary osteoprogenitor cellsosteoprogenitor cells comparedwnt16 knockdown culturessasp mrna expressionisolated osteoprogenitor cellsinduced sasp expressionassess cell senescenceseven disease controlosteoprogenitor cellswnt16 expressionisolated usingcell senescencewnt16 treatmentvitro modelvitro assaysstill unknownstained markedlypathophysiological changesp21 proteinoutgrowth methodidentified rolehighly expressedh2o2 stressforming activityfacet jointsconclusion wnt16ankylosing spondylitisalso validated
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