Additional file 2 of The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

Additional file 2: Figure S2. Individual pixel-pigment analysis of mucus staining of tissue samples revealed a distinctly higher abundance of pixels in the lower range of the pigment spectrum (darker colors, blue and purple) in GOS-fed old animals, compared to young or control-fed old animals (a). Pigment-specific histograms of the RGB image files were generated using ImageJ software, revealing differences between samples in the abundance of pixels in each image, as well as providing quantification of pixels within each pigment range (b). Ratios between epithelial and mucosal pixels were calculated and used in determining the fold change in pigment between specific pairs of animals, Old animals GOS vs Control diets, Young animals GOS vs Control diets, Old animals vs Young animals feeding on control diet, and finally Old animals vs Young animals feeding on GOS diet (c). Mucus fold change was calculated for each animal compared to Young animals fed control diet (d).