Additional file 2 of TLR7 is expressed by support cells, but not sensory neurons, in ganglia

Additional file 2: Figure S2. Immunohistochemistry with TLR7 antibodies indicates TLR-independent labeling of sensory neurons. Wild type and TLR7 knock out (KO) mouse dorsal root ganglia and vagal ganglia were paraffin embedded and processed for immunohistochemistry. Two separate TLR7 antibodies (TLR7 Ab-1, Novus; TLR7 Ab-2, Abcam) labeled small-to-medium-sized neurons (brown) in (A) dorsal root ganglia and (B) vagal ganglia in both wild type and TLR7 KO mice, and in (C) guinea pig dorsal root, nodose, and jugular ganglia. No primary antibody control experiments performed in parallel with TLR7 staining excluded non-specific secondary staining as the cause for neuronal labeling. Scale bar = 50 μm. Blue = hematoxylin nuclear stain. N = 4, representative images shown.