Additional file 2 of SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds

Li, Chao; Zong, Yuan; Jin, Shuai; Zhu, Haocheng; Lin, Dexing; Li, Shengnan; Qiu, Jin-Long; Wang, Yanpeng; Gao, Caixia

doi:10.6084/m9.figshare.12494756.v1

13059_2020_2051_MOESM2_ESM.docx (26.71 kB)

Additional file 2 of SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds

journal contribution

posted on 2020-06-17, 03:29 authored by Chao Li, Yuan Zong, Shuai Jin, Haocheng Zhu, Dexing Lin, Shengnan Li, Jin-Long Qiu, Yanpeng Wang, Caixia Gao

Additional file 2: Sequences S1. DNA sequences of the OsU3 and TaU6 promoters used in this study. Sequences S2. DNA sequences of sgRNA, esgRNA, and scRNAs used in this study. Sequences S3. DNA sequences of the modules composed of PBEcs and PABEcs used in this study. Sequences S4. DNA sequences of templates for assemble multiple sgRNAs used in this study. Sequences S5. DNA sequences of SWISSv2/v3 and SWISSv2/v3-NG used in this study.