Additional file 2 of NOTCH2 variant D1853H is mutated in two non-syndromic premature ovarian insufficiency patients from a Chinese pedigree

Li, Lin; Feng, Fan; Zhao, Minying; Li, Tengyan; Yue, Wentao; Ma, Xu; Wang, Binbin; Yin, Chenghong

doi:10.6084/m9.figshare.12160032.v1

13048_2020_645_MOESM2_ESM.pdf (601.21 kB)

Additional file 2 of NOTCH2 variant D1853H is mutated in two non-syndromic premature ovarian insufficiency patients from a Chinese pedigree

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posted on 2020-04-21, 03:53 authored by Lin Li, Fan Feng, Minying Zhao, Tengyan Li, Wentao Yue, Xu Ma, Binbin Wang, Chenghong Yin

Additional file 2 Fig. S2. Construction of plasmids containing the WT or mutant NOTCH2 intracellular domain (ICD). (A) the pHS-BVC-LW450 vector map. The vector contained a 3× flag and internal ribosome entry site (IRES) - enhanced green fluorescent protein (EGFP) sequences. (B) Sanger sequencing validated the WT and 5557C (mut) plasmid sequences.

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National Natural Science Foundation of China CAMS Innovation Fund for Medical Sciences CAMS Fund for Excellent Young Medical Talents Key Medical Science Research Projects of Hebei Province

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Premature ovarian insufficiency NOTCH2 Whole-exome sequencing RNA sequencing Gene ontology Collagen degradation

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Additional file 2 of NOTCH2 variant D1853H is mutated in two non-syndromic premature ovarian insufficiency patients from a Chinese pedigree

Funding

National Natural Science Foundation of China CAMS Innovation Fund for Medical Sciences CAMS Fund for Excellent Young Medical Talents Key Medical Science Research Projects of Hebei Province

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