Additional file 2 of Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and tropical ecosystems

Additional file 1: Table S1. Statistics of de novo assemblies and ARG-carrying contigs. Fig. S1. Geographical distribution of sampling sites. Fig. S2. Antibiotic resistance regulatory genes in soils with arlR, cpxR, ompR, vanR and vanS found in all 26 soils and part of shared background. Fig. S3. Venn diagram showing shared ARGs among Alaska, Midwest USA, and Amazon soils. Fig. S4. (A) The shared and exclusive ARGs between Amazon rainforest soils and pasture soils. (B) The composition of the exclusive ARGs. Fig. S5. ARG coverage (length of an assembled contig divided by length of the intact ARG) on de novo assemblies. Fig. S6. Network analysis assessing the ARG cluster across soils from tundra, temperate prairie and tropical ecosystems. Fig. S7. Pearson correlation between (A) ARG diversity and bacterial diversity; (B) resistome abundance and bacterial diversity. Fig. S8. Rank percentage of soil resistome abundance of top 50 ARGs. Fig. S9. Nonpareil curves showing estimated average coverage in soil datasets. Fig. S10. Per base coverage of vanS regulatory gene in 26 soils.