Additional file 2 of Long noncoding RNA TINCR facilitates hepatocellular carcinoma progression and dampens chemosensitivity to oxaliplatin by regulating the miR-195-3p/ST6GAL1/NF-κB pathway

Additional file 2: Figure S2. TINCR regulates HCC progression and oxaliplatin sensitivity through TINCR/miR-195-3p/ST6GAL1/NF kappa B Signaling. A, MiR-195-3p tends to be associated with good prognosis in the TCGA database. B-C, GSEA analysis based on TCGA data set shows a significant enrichment of high TINCR expression on gene sets related to several classical pathways (B), including NF kappa B pathway (C). D-E, Quantification of transwell migration (D) and invasion (E) assays in HepG2 and HuH7 cotransfected with si-TINCR 2# or scrambled control together with pc-DNA3.1-ST6GAL1 or empty vector. F, Representative images of the apoptosis assay of oxaliplatin (16 μg/ml) in the above-mentioned transfected HuH7 cells. G, 7 genes including ST6GAL1 are identified between the predicted target mRNAs in TargetScan and mRNAs that down-regulated with TINCR silencing in RNA-Seq. H, Western blot analysis of expression of key molecules in other typical pathways in HepG2 cells transfected with scrambled, si-ST6GAL1 or si-TINCR #2. I, Western blot analysis of expression of ST6GAL1 and NF-kappa-B-related markers in HepG2 cells cotransfected with pcDNA3.1-ST6GAL1 or empty vector and IκBα phosphorylation inhibitor (BAY 11–7085) or control.