Additional file 2 of Exposure to arsenic at different life-stages and DNA methylation meta-analysis in buccal cells and leukocytes

Additional file 2: Fig. S1: The leading principal components of the 5000 most variable CpG sites across the Chile study’s buccal cell and PBMC samples suggest that within-participant similarity is not a significant source of variability. Fig. S2: A permutation test of within-individual similarity scores identifies one nominally significant pair of samples (p < 0.05). After correcting for multiple testing (FDR < 0.05, FWER < 0.05), no samples are found to be significantly similar. Fig. S3: Q-Q plot of meta-analysis results of differentially methylated positions (DMPs) in PBMC EWAS (i.e., the Chile PBMC study, Bangladesh 450 K study, and Bangladesh 850 K study) adjusted for age, smoking status, cell type proportions, and sex in the Chile studies. Supplemental Table S1: Heterogeneity of differentially methylated positions (DMPs) and differentially variable positions (DVPs) in meta-analyses. DMPs and DVPs (FDR < 0.05) in the PBMC meta-analysis (i.e., including the Chile PBMC study, Bangladesh 450 K study, and Bangladesh 850 K study) and PBMC + buccal cell meta-analysis (i.e., including the Chile buccal cell study) adjusted for age, smoking status, cell type proportions, and sex (in the Chile studies). Supplemental Table S2: KEGG pathway analysis of differentially methylated regions (DMRs) and differentially variable regions (DVRs) in the PBMC meta-analysis (i.e., including the the Chile PBMC study, Bangladesh 450 K study, and Bangladesh 850 K study) and PBMC + buccal cell meta-analysis (i.e., including the Chile buccal cell study) adjusted for age, smoking status, cell type proportions, and sex (in the Chile studies). Pathways significant at p < 0.05 are shown. Supplemental Table S3: Summary of genes containing differentially methylated positions (DMPs) of differentially variable positions (DVPs) identified in meta-analyses in and previous EWAS.