13064_2020_141_MOESM2_ESM.pdf (314.01 kB)
Additional file 2 of Drosophila enabled promotes synapse morphogenesis and regulates active zone form and function
journal contribution
posted on 2020-03-18, 04:46 authored by Elizabeth M. McNeill, Cheryl Thompson, Brett Berke, Vivian T. Chou, Jannette Rusch, April Duckworth, Jamin DeProto, Alicia Taylor, Julie Gates, Frank Gertler, Haig Keshishian, David Van VactorAdditional file 2 Supplemental Figure 2. Structured illumination microscopy images (A-B) and quantification (C-D) of NMJs from muscle 6/7 in segment A2 of third-instar wandering larvae. Wild-type flies (control; A-A’) and enaLOF (B-B′) are shown stained with HRP (red) and Brp (green) staining. C, D, Reconstruction and quantification of images showed that the density of Brp puncta (puncta per bouton area) was unchanged compared to control (C). However, Brp puncta volume was very significantly increased in enaLOF compared to controls. ** P < 0.01, as determined by Welch’s test; error bars indicate ± s.e.m. of genotype; orange and blue shading indicate ± s.e.m. of muscle 4 and 6/7 controls, respectively; n ≥ 20 NMJs for all genotypes, scale = 1 μm.