40168_2021_1128_MOESM2_ESM.docx (3.5 MB)
Additional file 2 of Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri
journal contribution
posted on 2021-10-04, 03:08 authored by Hao-Yu Liu, Antoine Giraud, Cedric Seignez, David Ahl, Feilong Guo, John Sedin, Tomas Walden, Jee-Hwan Oh, Jan Peter van Pijkeren, Lena Holm, Stefan Roos, Stefan Bertilsson, Mia PhillipsonAdditional file 1: Fig. S1. The staining and gating strategy of cell subsets isolated from Peyer’s patches and in vivo imaging of whole tissues. Fig. S2. Description of B cell identity and features of large B and small B cells in PPs. Fig. S3. Changes of lymphocyte populations in response to Limosilactobacillus reuteri strains and FTY720-treatment. Fig. S4. Effects of L. reuteri strains on B cell-IgA, colonic microbiota and T cell responses. Fig. S5. Effects of L. reuteri and/or DSS on the intestine. Table S1. Enrichment of gene ontology categories in large B cells versus small B cells. Table S2. Gene ontology enrichment score (functional cluster analysis) of highly repressed genes by L. reuteri-treatment in large-B compared to large-B cells from control. Table S3. Primers used for quantitative-RT-PCR and oligonucleotides. Table S4. Histological scoring system for DSS induced ileal disruption.
