13007_2020_638_MOESM2_ESM.docx (94.96 kB)
Additional file 2 of Anthocyanin, a novel and user-friendly reporter for convenient, non-destructive, low cost, directly visual selection of transgenic hairy roots in the study of rhizobia-legume symbiosis
journal contribution
posted on 2020-07-07, 05:04 authored by Yinglun Fan, Xiuyuan Wang, Haiyun Li, Shuang Liu, Liangshen Jin, Yanyan Lyu, Mengdi Shi, Sirui Liu, Xinyue Yang, Shanhua LyuAdditional file 2: Fig. S1. Transcription analysis of AtMyb75 in induced soybean hairy roots by RT-PCR. RNAs were extracted from independent hairy roots induced by A. rhizogenes carrying p35AtM75 construct. Gm-Actin (27 cycles) (a) and AtMyb75 (30 cycles) (b) were amplified, respectively. Lane 1–4, non-transgenic white root inoculated with K599-p35AtM75; lane 5, p35AtM75 plasmid; lane 6–11, independent transgenic root with anthocyanin accumulation inoculated with K599-p35AtM75; lane 12, K599; lane 13, ddH2O. M, DL2000 DNA ladder bought from Sangon Biotech (band is 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 2000 bp, respectively).