Springer Nature
Browse
13007_2020_638_MOESM2_ESM.docx (94.96 kB)

Additional file 2 of Anthocyanin, a novel and user-friendly reporter for convenient, non-destructive, low cost, directly visual selection of transgenic hairy roots in the study of rhizobia-legume symbiosis

Download (94.96 kB)
journal contribution
posted on 2020-07-07, 05:04 authored by Yinglun Fan, Xiuyuan Wang, Haiyun Li, Shuang Liu, Liangshen Jin, Yanyan Lyu, Mengdi Shi, Sirui Liu, Xinyue Yang, Shanhua Lyu
Additional file 2: Fig. S1. Transcription analysis of AtMyb75 in induced soybean hairy roots by RT-PCR. RNAs were extracted from independent hairy roots induced by A. rhizogenes carrying p35AtM75 construct. Gm-Actin (27 cycles) (a) and AtMyb75 (30 cycles) (b) were amplified, respectively. Lane 1–4, non-transgenic white root inoculated with K599-p35AtM75; lane 5, p35AtM75 plasmid; lane 6–11, independent transgenic root with anthocyanin accumulation inoculated with K599-p35AtM75; lane 12, K599; lane 13, ddH2O. M, DL2000 DNA ladder bought from Sangon Biotech (band is 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 2000 bp, respectively).

Funding

National Natural Science Foundation of China Natural Science Foundation of Shandong Province

History

Usage metrics

    Plant Methods

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC